Antigenic analysis of coccidioidin and spherulin determined by two-dimensional immunoelectrophoresis
نویسندگان
چکیده
منابع مشابه
Antigenic identity of biologically active antigens in coccidioidin and spherulin.
We recently reported the isolation of three clinically relevant antigens from coccidioidin; viz., the antigen that is reactive in the immunodiffusion (ID) assay for detecting tube precipitin (TP) antibody (designated IDTP); the antigen that is reactive in the ID assay for detecting complement-fixing antibody (designated IDCF); and the heat-stable (HS) antigen which, when demonstrated in soluble...
متن کاملDermal sensitivity to different doses of spherulin and coccidioidin.
Spherotin, a new reagent, was compared with coccididdin in subjects nonreactive to the standnrd dew of either refor eliciting delayed dermal hypersensitivity in 298 human agent. Severe local reactions were encountered in only subjects living in an area endemic for Coccidioides imooe subM these reactions were produced by the low mi& As standard (dilute) doses, spherulin detected 32 doses of both...
متن کاملCharacterization and comparison of mycobacterial antigens by two-dimensional immunoelectrophoresis.
Two-dimensional immunoelectrophoresis (2D-IEP), in which a complex of antigens is subjected to electrophoresis first through an agarose matrix in one direction and secondly through an antiserum-agarose matrix at right angles to the first direction, was evaluated as a tool for analysis of mycobacterial antigens. Cell extracts from four species of mycobacteria, Mycobacterium tuberculosis (four st...
متن کاملa comparative analysis of the marginal microleakages of two pit and fissure sealans, conseal-f and conseal clear
چکیده ندارد.
15 صفحه اولComparison of coccidioidin and spherulin in complement fixation tests for coccidioidomycosis.
Coccidioidin, an extract from the saprophytic mycelial form of Coccidioides immitis, has been a very useful antigen preparation in serological tests for coccidioidomycosis. Its sensitivity has been very good for detecting most types of clinical disease, but tests with coccidioidin have been negative for 40% or more of patients with chronic pulmonary disease, the clinical entity which must be di...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1978
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.20.2.541-551.1978